CCL-153 HFL1 人肺成纖維細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！

ATCC® Number: CCL-153™

Designations: HFL1

Depositors: RG Crystal

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: fibroblast

CCL-153 HFL1 人肺成纖維細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

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Reverse Transcript: negative

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11,12

D16S539: 9,11

D5S818: 12

D7S820: 9,10

THO1: 7,9

TPOX: 6,9

vWA: 17

Cytogenetic Analysis: normal human; diploid; stable

Isoenzymes: G6PD, B

Age: fetus

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004

recommended serum:ATCC 30-2020

References: 23400: Breul SD, et al. Control of collagen production by human diploid lung fibroblasts. J. Biol. Chem. 255: 5250-5260, 1980. PubMed: 7372635

CCL-153 HFL1